Faculty
Oncology Division
Alphabetical list (active faculty):   
David H. Spencer

David H. Spencer, MD, PhD

Assistant Professor

Department of Medicine

Oncology Division

Stem Cell Biology

Research Interests

  • AML
  • Cancer
  • Epigenetics
  • DNA methylation
  • Genomics

Contact

  • 314-273-0739 (office)
  • 314-362-9333 (fax)
  • Division of Oncology
    Campus Box 8056
    Washington University
    660 South Euclid Avenue
    St. Louis, MO 63110
  • Room 619 Southwest Tower (office)

Original Articles

  • DNMT3A Overgrowth Syndrome Is Associated With the Development of Hematopoietic Malignancies in Children and Young Adults
    Ferris MA, Smith AM, Heath SE, Duncavage EJ, Oberley MJ, Freyer D, Wynn R, Douzgou S, Maris JM, Reilly AF, Wu M, Choo F, Fiets RB, Koene S, Spencer DH, Miller CA, Shinawi M, Ley TJ
    Blood 2021 Nov 17; [Epub ahead of print]
  • Genome Sequencing in Myeloid Cancers. Reply
    Duncavage EJ, Schroeder MC, Spencer DH
    N Engl J Med 2021 Jun 24;384(25):e106
  • Heterozygous Variants of CLPB Are a Cause of Severe Congenital Neutropenia
    Warren JT, Cupo RR, Wattanasirakul P, Spencer D, Locke AE, Makaryan V, Bolyard AA, Kelley ML, Kingston NL, Shorter J, Bellanne-Chantelot C, Donadieu J, Dale DC, Link DC
    Blood 2021 Jun 11; [Epub ahead of print]
  • Impact of a 40-Gene Targeted Panel Test on Physician Decision Making for Patients With Acute Myeloid Leukemia
    Barnell EK, Newcomer KF, Skidmore ZL, Krysiak K, Anderson SR, Wartman LD, Oh ST, Welch JS, Stockerl-Goldstein KE, Vij R, Cashen AF, Pusic I, Westervelt P, Abboud CN, Ghobadi A, Uy GL, Schroeder MA, DiPersio JF, Politi MC, Spencer DH, Duncavage EJ, Ley TJ, Griffith M, Jacoby MA, Griffith OL
    JCO Precis Oncol 2021 Jan 14;5
  • Genome Sequencing as an Alternative to Cytogenetic Analysis in Myeloid Cancers
    Duncavage EJ, Schroeder MC, O'Laughlin M, Wilson R, MacMillan S, Bohannon A, Kruchowski S, Garza J, Du F, Hughes AEO, Robinson J, Hughes E, Heath SE, Baty JD, Neidich J, Christopher MJ, Jacoby MA, Uy GL, Fulton RS, Miller CA, Payton JE, Link DC, Walter MJ, Westervelt P, DiPersio JF, Ley TJ, Spencer DH
    N Engl J Med 2021 Mar 11;384(10):924-935
  • Contribution of CTCF Binding to Transcriptional Activity at the HOXA Locus in NPM1-Mutant AML Cells
    Ghasemi R, Struthers H, Wilson ER, Spencer DH
    Leukemia 2021 Feb;35(2):404-416
  • Deletions in BCP-ALL Result in a TAD More FLT3
    Spencer DH
    Blood 2020 Aug 20;136(8):919-920
  • Remethylation of Dnmt3a (-/-) Hematopoietic Cells Is Associated With Partial Correction of Gene Dysregulation and Reduced Myeloid Skewing
    Ketkar S, Verdoni AM, Smith AM, Bangert CV, Leight ER, Chen DY, Brune MK, Helton NM, Hoock M, George DR, Fronick C, Fulton RS, Ramakrishnan SM, Chang GS, Petti AA, Spencer DH, Miller CA, Ley TJ
    Proc Natl Acad Sci U S A 2020 Feb 11;117(6):3123-3134
  • Inflammatory Cytokines Promote Clonal Hematopoiesis With Specific Mutations in Ulcerative Colitis Patients
    Zhang CRC, Nix D, Gregory M, Ciorba MA, Ostrander EL, Newberry RD, Spencer DH*, Challen GA* (*Shared authorship )
    Exp Hematol 2019 Dec;80:36-41e3
  • Immunological Ignorance Is an Enabling Feature of the Oligo-Clonal T Cell Response to Melanoma Neoantigens
    Linette GP, Becker-Hapak M, Skidmore ZL, Baroja ML, Xu C, Hundal J, Spencer DH, Fu W, Cummins C, Robnett M, Kaabinejadian S, Hildebrand WH, Magrini V, Demeter R, Krupnick AS, Griffith OL, Griffith M, Mardis ER, Carreno BM
    Proc Natl Acad Sci U S A 2019 Nov 19;116(47):23662-23670
  • Functional Characterization of Biallelic RTTN Variants Identified in an Infant With Microcephaly, Simplified Gyral Pattern, Pontocerebellar Hypoplasia, and Seizures
    Wambach JA, Wegner DJ, Yang P, Shinawi M, Baldridge D, Betleja E, Shimony JS, Spencer D, Hackett BP, Andrews MV, Ferkol T, Dutcher SK, Mahjoub MR, Cole FS
    Pediatr Res 2018 Sep;84(3):435-441
  • DNMT3A(R882)-Associated Hypomethylation Patterns Are Maintained in Primary AML Xenografts, but Not in the DNMT3A(R882C) OCI-AML3 Leukemia Cell Line
    Chen D, Christopher M, Helton NM, Ferguson I, Ley TJ, Spencer DH
    Blood Cancer J 2018 Apr 4;8(4):38
  • Sequencing of Tumor DNA to Guide Cancer Risk Assessment and Therapy
    Spencer DH, Ley TJ
    JAMA 2018 Apr 10;319(14):1497-1498
  • Expression Profiling of snoRNAs in Normal Hematopoiesis and AML
    Warner WA*, Spencer DH*, Trissal M, White BS, Helton N, Ley TJ, Link DC (*Shared authorship)
    Blood Adv 2018 Jan 23;2(2):151-163
  • CIViC Is a Community Knowledgebase for Expert Crowdsourcing the Clinical Interpretation of Variants in Cancer
    Griffith M, Spies NC, Krysiak K, McMichael JF, Coffman AC, Danos AM, Ainscough BJ, Ramirez CA, Rieke DT, Kujan L, Barnell EK, Wagner AH, Skidmore ZL, Wollam A, Liu CJ, Jones MR, Bilski RL, Lesurf R, Feng YY, Shah NM, Bonakdar M, Trani L, Matlock M, Ramu A, Campbell KM, Spies GC, Graubert AP, Gangavarapu K, Eldred JM, Larson DE, Walker JR, Good BM, Wu C, Su AI, Dienstmann R, Margolin AA, Tamborero D, Lopez-Bigas N, Jones SJ, Bose R, Spencer DH, Wartman LD, Wilson RK, Mardis ER, Griffith OL
    Nat Genet 2017 Jan 31;49(2):170-174
  • Comprehensive Discovery of Noncoding RNAs in Acute Myeloid Leukemia Cell Transcriptomes
    Zhang J, Griffith M, Miller CA, Griffith OL, Spencer DH, Walker JR, Magrini V, McGrath SD, Ly A, Helton NM, Trissal M, Link DC, Dang HX, Larson DE, Kulkarni S, Cordes MG, Fronick CC, Fulton RS, Klco JM, Mardis ER, Ley TJ, Wilson RK, Maher CA
    Exp Hematol 2017 Nov;55:19-33
  • CpG Island Hypermethylation Mediated by DNMT3A Is a Consequence of AML Progression
    Spencer DH, Russler-Germain DA, Ketkar S, Helton NM, Lamprecht TL, Fulton RS, Fronick CC, O'Laughlin M, Heath SE, Shinawi M, Westervelt P, Payton JE, Wartman LD, Welch JS, Wilson RK, Walter MJ, Link DC, DiPersio JF, Ley TJ
    Cell 2017 Feb 23;168(5):801-816e13
  • Occult Specimen Contamination in Routine Clinical Next-Generation Sequencing Testing
    Sehn JK, Spencer DH, Pfeifer JD, Bredemeyer AJ, Cottrell CE, Abel HJ, Duncavage EJ
    Am J Clin Pathol 2015 Oct;144(4):667-74
  • Clinical Next-Generation Sequencing in Patients With Non-Small Cell Lung Cancer
    Hagemann IS, Devarakonda S, Lockwood CM, Spencer DH, Guebert K, Bredemeyer AJ, Al-Kateb H, Nguyen TT, Duncavage EJ, Cottrell CE, Kulkarni S, Nagarajan R, Seibert K, Baggstrom M, Waqar SN, Pfeifer JD, Morgensztern D, Govindan R
    Cancer 2015 Feb 15;121(4):631-9
  • Association Between Mutation Clearance After Induction Therapy and Outcomes in Acute Myeloid Leukemia
    Klco JM, Miller CA, Griffith M, Petti A, Spencer DH, Ketkar-Kulkarni S, Wartman LD, Christopher M, Lamprecht TL, Helton NM, Duncavage EJ, Payton JE, Baty J, Heath SE, Griffith OL, Shen D, Hundal J, Chang GS, Fulton R, O'Laughlin M, Fronick C, Magrini V, Demeter RT, Larson DE, Kulkarni S, Ozenberger BA, Welch JS, Walter MJ, Graubert TA, Westervelt P, Radich JP, Link DC, Mardis ER, DiPersio JF, Wilson RK, Ley TJ
    JAMA 2015 Aug 25;314(8):811-22
  • Epigenomic Analysis of the HOX Gene Loci Reveals Mechanisms That May Control Canonical Expression Patterns in AML and Normal Hematopoietic Cells
    Spencer DH, Young MA, Lamprecht TL, Helton NM, Fulton R, O'Laughlin M, Fronick C, Magrini V, Demeter RT, Miller CA, Klco JM, Wilson RK, Ley TJ
    Leukemia 2015 Jun;29(6):1279-89
  • The R882H DNMT3A Mutation Associated With AML Dominantly Inhibits Wild-Type DNMT3A by Blocking Its Ability to Form Active Tetramers
    Russler-Germain DA, Spencer DH, Young MA, Lamprecht TL, Miller CA, Fulton R, Meyer MR, Erdmann-Gilmore P, Townsend RR, Wilson RK, Ley TJ
    Cancer Cell 2014 Apr 14;25(4):442-54
  • Functional Heterogeneity of Genetically Defined Subclones in Acute Myeloid Leukemia
    Klco JM*, Spencer DH*, Miller CA, Griffith M, Lamprecht TL, O'Laughlin M, Fronick C, Magrini V, Demeter RT, Fulton RS, Eades WC, Link DC, Graubert TA, Walter MJ, Mardis ER, DiPersio JF, Wilson RK, Ley TJ (*shared authorship)
    Cancer Cell 2014 Mar 17;25(3):379-92
  • Performance of Common Analysis Methods for Detecting Low-Frequency Single Nucleotide Variants in Targeted Next-Generation Sequence Data
    Spencer DH, Tyagi M, Vallania F, Bredemeyer AJ, Pfeifer JD, Mitra RD, Duncavage EJ
    J Mol Diagn 2014 Jan;16(1):75-88
  • Validation of a Next-Generation Sequencing Assay for Clinical Molecular Oncology
    Cottrell CE, Al-Kateb H, Bredemeyer AJ, Duncavage EJ, Spencer DH, Abel HJ, Lockwood CM, Hagemann IS, O'Guin SM, Burcea LC, Sawyer CS, Oschwald DM, Stratman JL, Sher DA, Johnson MR, Brown JT, Cliften PF, George B, McIntosh LD, Shrivastava S, Nguyen TT, Payton JE, Watson MA, Crosby SD, Head RD, Mitra RD, Nagarajan R, Kulkarni S, Seibert K, Virgin HW 4th, Milbrandt J, Pfeifer JD
    J Mol Diagn 2014 Jan;16(1):89-105
  • Clinical Genomicist Workstation
    Sharma MK, Phillips J, Agarwal S, Wiggins WS, Shrivastava S, Koul SB, Bhattacharjee M, Houchins CD, Kalakota RR, George B, Meyer RR, Spencer DH, Lockwood CM, Nguyen TT, Duncavage EJ, Al-Kateb H, Cottrell CE, Godala S, Lokineni R, Sawant SM, Chatti V, Surampudi S, Sunkishala RR, Darbha R, Macharla S, Milbrandt JD, Virgin HW, Mitra RD, Head RD, Kulkarni S, Bredemeyer A, Pfeifer JD, Seibert K, Nagarajan R
    AMIA Jt Summits Transl Sci Proc 2013 Mar 18;2013:156-7
  • Comparison of Clinical Targeted Next-Generation Sequence Data From Formalin-Fixed and Fresh-Frozen Tissue Specimens
    Spencer DH, Sehn JK, Abel HJ, Watson MA, Pfeifer JD, Duncavage EJ
    J Mol Diagn 2013 Sep;15(5):623-33
  • Genomic and Epigenomic Landscapes of Adult De Novo Acute Myeloid Leukemia
    Ley TJ, Miller C, Ding L, Raphael BJ, Mungall AJ, Robertson A, Hoadley K, Triche TJ Jr, Laird PW, Baty JD, Fulton LL, Fulton R, Heath SE, Kalicki-Veizer J, Kandoth C, Klco JM, Koboldt DC, Kanchi KL, Kulkarni S, Lamprecht TL, Larson DE, Lin L, Lu C, McLellan MD, McMichael JF, Payton J, Schmidt H, Spencer DH, Tomasson MH, Wallis JW, Wartman LD, Watson MA, Welch J, Wendl MC, Ally A, Balasundaram M, Birol I, Butterfield Y, Chiu R, Chu A, Chuah E, Chun HJ, Corbett R, Dhalla N, Guin R, He A, Hirst C, Hirst M, Holt RA, Jones S, Karsan A, Lee D, Li HI, Marra MA, Mayo M, Moore RA, Mungall K, Parker J, Pleasance E, Plettner P, Schein J, Stoll D, Swanson L, Tam A, Thiessen N, Varhol R, Wye N, Zhao Y, Gabriel S, Getz G, Sougnez C, Zou L, Leiserson MD, Vandin F, Wu HT, Applebaum F, Baylin SB, Akbani R, Broom BM, Chen K, Motter TC, Nguyen K, Weinstein JN, Zhang N, Ferguson ML, Adams C, Black A, Bowen J, Gastier-Foster J, Grossman T, Lichtenberg T, Wise L, Davidsen T, Demchok JA, Shaw KR, Sheth M, Sofia HJ, Yang L, Downing JR, Eley G
    Cancer Genome Atlas Research Network

    N Engl J Med 2013 May 30;368(22):2059-74
  • Detection of FLT3 Internal Tandem Duplication in Targeted, Short-Read-Length, Next-Generation Sequencing Data
    Spencer DH, Abel HJ, Lockwood CM, Payton JE, Szankasi P, Kelley TW, Kulkarni S, Pfeifer JD, Duncavage EJ
    J Mol Diagn 2013 Jan;15(1):81-93
  • Genomic Impact of Transient Low-Dose Decitabine Treatment on Primary AML Cells
    Klco JM*, Spencer DH*, Lamprecht TL, Sarkaria SM, Wylie T, Magrini V, Hundal J, Walker J, Varghese N, Erdmann-Gilmore P, Lichti CF, Meyer MR, Townsend RR, Wilson RK, Mardis ER, Ley TJ (*shared authorship)
    Blood 2013 Feb 28;121(9):1633-43
  • Direct-To-Consumer Genetic Testing: Reliable or Risky?
    Spencer DH, Lockwood C, Topol E, Evans JP, Green RC, Mansfield E, Tezak Z
    Clin Chem 2011 Dec;57(12):1641-4
  • Validation and Implementation of the GeneXpert MRSA/SA Blood Culture Assay in a Pediatric Setting
    Spencer DH, Sellenriek P, Burnham CA
    Am J Clin Pathol 2011 Nov;136(5):690-4
  • Red Cell Transfusion Decreases Hemoglobin A1c in Patients With Diabetes
    Spencer DH, Grossman BJ, Scott MG
    Clin Chem 2011 Feb;57(2):344-6
  • IFRD1 Is a Candidate Gene for SMNA on Chromosome 7q22-q23
    Brkanac Z, Spencer D, Shendure J, Robertson PD, Matsushita M, Vu T, Bird TD, Olson MV, Raskind WH
    Am J Hum Genet 2009 May;84(5):692-7
  • Growth Phenotypes of Pseudomonas Aeruginosa LasR Mutants Adapted to the Airways of Cystic Fibrosis Patients
    D'Argenio DA, Wu M, Hoffman LR, Kulasekara HD, Deziel E, Smith EE, Nguyen H, Ernst RK, Larson Freeman TJ, Spencer DH, Brittnacher M, Hayden HS, Selgrade S, Klausen M, Goodlett DR, Burns JL, Ramsey BW, Miller SI
    Mol Microbiol 2007 Apr;64(2):512-33
  • Detecting Disease-Causing Mutations in the Human Genome by Haplotype Matching
    Spencer DH, Bubb KL, Olson MV
    Am J Hum Genet 2006 Nov;79(5):958-64
  • Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas Aeruginosa
    Smith EE, Sims EH, Spencer DH, Kaul R, Olson MV
    J Bacteriol 2005 Mar;187(6):2138-47
  • Genome Mosaicism Is Conserved but Not Unique in Pseudomonas Aeruginosa Isolates From the Airways of Young Children With Cystic Fibrosis
    Ernst RK, D'Argenio DA, Ichikawa JK, Bangera MG, Selgrade S, Burns JL, Hiatt P, McCoy K, Brittnacher M, Kas A, Spencer DH, Olson MV, Ramsey BW, Lory S, Miller SI
    Environ Microbiol 2003 Dec;5(12):1341-9
  • Comprehensive Transposon Mutant Library of Pseudomonas Aeruginosa
    Jacobs MA, Alwood A, Thaipisuttikul I, Spencer D, Haugen E, Ernst S, Will O, Kaul R, Raymond C, Levy R, Chun-Rong L, Guenthner D, Bovee D, Olson MV, Manoil C
    Proc Natl Acad Sci U S A 2003 Nov 25;100(24):14339-44
  • Whole-Genome Sequence Variation Among Multiple Isolates of Pseudomonas Aeruginosa
    Spencer DH, Kas A, Smith EE, Raymond CK, Sims EH, Hastings M, Burns JL, Kaul R, Olson MV
    J Bacteriol 2003 Feb;185(4):1316-25
  • Genetic Variation at the O-Antigen Biosynthetic Locus in Pseudomonas Aeruginosa
    Raymond CK, Sims EH, Kas A, Spencer DH, Kutyavin TV, Ivey RG, Zhou Y, Kaul R, Clendenning JB, Olson MV
    J Bacteriol 2002 Jul;184(13):3614-22
  • Complete Genome Sequence of Pseudomonas Aeruginosa PAO1, an Opportunistic Pathogen
    Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV
    Nature 2000 Aug 31;406(6799):959-64